علوم زیستی دریا
Hassan Morovvati; Mahmood Khaksary Mahabady; Mehrzad Mesbah; Mehdi Hadi Jafari
Abstract
Barbus grypus is native fishes in Khuzestan province which its species with a wide distribution in the rivers that considered fisheries research in province. The fish have no stomach, space of intestine is narrow to store of food, for this reason anterior intestine dilated bowel and called intestinal ...
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Barbus grypus is native fishes in Khuzestan province which its species with a wide distribution in the rivers that considered fisheries research in province. The fish have no stomach, space of intestine is narrow to store of food, for this reason anterior intestine dilated bowel and called intestinal bulb. Study of histological structure of intestinal bulb in fishes can help to nutrition and food needs so that we can extend of this species. In this study, 10 adults Barbus grypus of both sexes with average weigh 354.25 ± 60.52 g and average length 36.25 ±4.16 cm was chosen, and after easy drawing, samples were removed from intestinal bulb. The 0.5cm were fixed by Bouin´s fixative soluble and 5-6µ thickness sections were made by paraffin embedding method and were stained by H&E and PAS. The histomorphometrical studies were done using digital Dino-Lite lens and Dino-capture1 software. The results showed that the number of goblet cells in the intestine increased toward the end of the intestinal bulb. The intestinal epithelium height in the anterior part of the intestinal bulb was higher than other parts. Muscular thickness was different in different parts of the intestinal bulb, the middle part of intestinal bulb has thickest muscle between other parts of intestinal bulb. The results of this research indicate that there is some analog histological structure of the intestinal bulb in Barbus grypus to compare of other same family: from wall diameter and intestinal epithelium height and diameter of mucosa.
علوم زیستی دریا
Hassan Morovvati; Mahmood Khaksary Mahabady; Mehrzad Mesbah; Reza Fakoori
Abstract
At this study, kidneys of 10 normal fishes have been studied macroscopic and microscopically. The kidneys were collected by autopsy and tissue samples were sectioned by routine paraffin embedding and stained by H&E and PAS. Macroscopic observation showed that the kidney in Barbus grypus situated ...
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At this study, kidneys of 10 normal fishes have been studied macroscopic and microscopically. The kidneys were collected by autopsy and tissue samples were sectioned by routine paraffin embedding and stained by H&E and PAS. Macroscopic observation showed that the kidney in Barbus grypus situated in the ventral region of vertebral column. The kidney could be divided into; head, body and tail portions according to their relative position. Microscopic studies showed that the kidneys consisted of excretory portion and hematopoietic-lymphoid tissues. Anterior portion of kidney (head) had more Hematopoietic-lymphoid tissues than the posterior portion (body and tail). Hematopoietic-lymphoid tissue consisted of cells which were found in different stages of development to blood cells. Excretory portion of kidney parenchyma consisted of renal corpuscle and urinary tubules. In microscopic section of glomerulus, endothelial and mesangial cells and nucleated red blood cells were seen. The number of renal corpuscles in the posterior portion of kidney was more than the anterior portion significantly, while there was no significant difference between the average diameter of renal corpuscles in the head, body and tail portions of kidney. Urinary tubules consisted of neck segment, proximal tubule (first and second portion), intermediate segment and distal segment. The collecting ducts were situated after the distal segments. No significant difference was observed in the measured parameters between the males and females fishes in this study. Based on the results of this study, the kidney of Barbus grypus is very similar to other species of the family Cyprinidae, histologically and anatomically.